The post-translational addition of a functional group covalently bonded to the protein are common to biologic drug substances, which can affect the structure, stability, function, and protein interaction. N-acetylation is one of the post-translational modifications (PTMs), which append an acetyl group to the N-terminal amino group. N-acetylation degree might alter the charge, hydrophobicity, lifespan, folding characteristics and binding properties, and polymorphism in drug metabolism. To confirm protein identity, ensure product quality and consistent manufacturing process, and avoid a potentially harmful side effect and low efficacy in the patients, N-acetylation analysis is an important biochemical technique during the processes of drug discovery. It can be used to analyze the N-terminal modification of biological agents or target proteins. n acetyl https://www.creative-proteomics.com/pronalyse/n-acetylation-analysis.html