Chromatography is a process for detaching the individual components of a mixture which according to their relative affinities for the stationary and mobile phases. The one who discover chromatography passed through a column of solid adsorbents, he coined the term chromatography, to represent the separation of color compounds. Column chromatography, the most commonly one, of which the stationary phase is put into a column through which the mobile phase is pumped. how to calculate protein purity https://www.creative-proteomics.com/pronalyse/protein-purity-analysis-hplc.html